Hotspot

trnE-UUC-trnT-GGU

LSC · 33308-34125 · 818 bp

Back to Vesalea

Pi 0.0139

MarkerSeek score 69.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 17
pi: 0.0139
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AACTTCCTTTCGCAGTACCC	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	962-1027 bp	1.0	81.2
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	917-982 bp	1.0	80.7
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	896-961 bp	1.0	80.6
trnE-UUC-trnT-GGU_p4	AACTTCCTTTCGCAGTACCC	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	959-1024 bp	1.0	80.0
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	914-979 bp	1.0	79.5