Hotspot

trnC-GCA-petN

LSC · 29524-30395 · 872 bp

Back to Vesalea

Pi 0.0164

MarkerSeek score 64.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 18
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	935-937 bp	1.0	82.2
trnC-GCA-petN_p2	GTATCAATTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	987-989 bp	1.0	81.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	953-955 bp	1.0	79.9
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TTAGAGCCCACTTCTTCCCC	50.0	55.0	58.1	58.7	998-1000 bp	1.0	77.4
trnC-GCA-petN_p5	GTATCAATTTGGCGGCATGG	TTAGAGCCCACTTCTTCCCC	50.0	55.0	57.9	58.7	1050-1052 bp	1.0	76.8