Hotspot

trnH-GUG-psbA

LSC · 246-602 · 357 bp

Back to Vesalea

Pi 0.0651

MarkerSeek score 77.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 27
pi: 0.0651
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GGATCAAGGCAGTGGATTGT	55.0	50.0	58.1	57.9	182-191 bp	1.0	87.9
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TGGATCAAGGCAGTGGATTG	55.0	50.0	58.1	57.9	183-192 bp	1.0	87.8
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GTGGATCAAGGCAGTGGATT	55.0	50.0	58.1	57.9	184-193 bp	1.0	87.8
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GATTGTGAATCCACCATGCG	55.0	50.0	58.1	57.5	168-177 bp	1.0	85.6
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GATTGTGAATCCACCATGCG	50.0	50.0	57.9	57.5	182-191 bp	1.0	85.1