primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GGATCAAGGCAGTGGATTGT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.263709	246	365	182-191	1.000000	87.9
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TGGATCAAGGCAGTGGATTG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.262201	246	365	183-192	1.000000	87.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	GTGGATCAAGGCAGTGGATT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.263709	246	365	184-193	1.000000	87.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	GATTGTGAATCCACCATGCG	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.609144	246	365	168-177	1.000000	85.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GATTGTGAATCCACCATGCG	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.613505	246	365	182-191	1.000000	85.1
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	14741	15921	1269-1294	1.000000	77.1
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.9	58.5	0.0	0.0	0.666168	14741	15921	1285-1310	1.000000	76.2
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.2	58.5	1.4	0.0	0.701648	14741	15921	1270-1295	1.000000	75.9
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.732109	14741	15921	1284-1309	1.000000	75.7
atpH-atpI_p5	atpH-atpI	5	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	20	20	45.0	50.0	58.0	57.3	1.4	14.7	0.774530	14741	15921	1254-1279	1.000000	75.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	29524	30395	935-937	1.000000	82.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	GTATCAATTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.9	58.2	14.3	0.0	0.289944	29524	30395	987-989	1.000000	81.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	29524	30395	953-955	1.000000	79.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	TTAGAGCCCACTTCTTCCCC	20	20	50.0	55.0	58.1	58.7	0.0	0.0	0.795880	29524	30395	998-1000	1.000000	77.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	GTATCAATTTGGCGGCATGG	TTAGAGCCCACTTCTTCCCC	20	20	50.0	55.0	57.9	58.7	14.3	0.0	0.852348	29524	30395	1050-1052	1.000000	76.8
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AACTTCCTTTCGCAGTACCC	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.186403	33308	34125	962-1027	1.000000	81.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33308	34125	917-982	1.000000	80.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	33308	34125	896-961	1.000000	80.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AACTTCCTTTCGCAGTACCC	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.331336	33308	34125	959-1024	1.000000	80.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	58.3	58.2	0.0	0.0	0.414292	33308	34125	914-979	1.000000	79.5
psaA-ycf3_p1	psaA-ycf3	1	CCAGGTTTAGCCCATTCCTC	ATCAAGCCGCTGAGTATTGG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.099551	44052	44864	985-1006	1.000000	81.8
psaA-ycf3_p2	psaA-ycf3	2	CCAGGTTTAGCCCATTCCTC	GGAAACAGGCTATAGCGCTT	20	20	55.0	50.0	57.9	58.0	0.0	18.7	0.103666	44052	44864	967-988	1.000000	81.8
psaA-ycf3_p3	psaA-ycf3	3	CCAGGTTTAGCCCATTCCTC	TTGGTTGAAGATCACCAGGC	20	20	55.0	50.0	57.9	58.1	0.0	3.2	0.148893	44052	44864	918-939	1.000000	81.6
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	44052	44864	958-979	1.000000	81.4
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	44052	44864	976-997	1.000000	81.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48726	49488	801-813	1.000000	81.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48726	49488	809-821	1.000000	80.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48726	49488	833-845	1.000000	80.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48726	49488	841-853	1.000000	80.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	48726	49488	815-827	1.000000	79.6
accD_p1	accD	1	ACGACGGTTGTCCTATTTGG	CTTAGTCCACACCTGTGCTC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.175004	60576	60725	791-995	1.000000	88.9
accD_p2	accD	2	ACGACGGTTGTCCTATTTGG	ACACCTGTGCTCGAATTTCA	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.343495	60576	60725	783-987	1.000000	87.6
accD_p3	accD	3	GAGCACAGGTGTGGACTAAG	ACACCTGTGCTCGAATTTCA	20	20	55.0	45.0	57.9	57.7	12.0	0.0	0.340600	60576	60725	991-1195	1.000000	86.8
accD_p4	accD	4	TGAAATTCGAGCACAGGTGT	CTTAGTCCACACCTGTGCTC	20	20	45.0	55.0	57.7	57.9	0.9	0.0	0.340600	60576	60725	1007-1211	1.000000	86.7
accD_p5	accD	5	CGACGGTTGTCCTATTTGGA	CTTAGTCCACACCTGTGCTC	20	20	50.0	55.0	57.6	57.9	0.0	0.0	0.459595	60576	60725	790-994	1.000000	86.6
accD-psaI_p1	accD-psaI	1	TCAATCTAGTCGTACCACGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	55.7	58.0	0.0	26.9	2.281863	63141	63733	747-767	1.000000	69.9
accD-psaI_p2	accD-psaI	2	TCAATCTAGTCGTACCACGT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	55.7	57.9	0.0	26.8	2.358397	63141	63733	753-773	1.000000	69.2
accD-psaI_p3	accD-psaI	3	TCAGTTCCATGGTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	22	20	40.9	45.0	57.5	58.0	12.6	26.9	2.480483	63141	63733	695-715	1.000000	68.6
accD-psaI_p4	accD-psaI	4	TTCAATCTAGTCGTACCACGT	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	56.5	58.0	0.0	26.9	2.505547	63141	63733	748-768	1.000000	68.1
accD-psaI_p5	accD-psaI	5	TCAGTTCCATGGTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	22	20	40.9	45.0	57.5	57.9	12.6	26.8	2.557017	63141	63733	701-721	1.000000	67.9
clpP_p1	clpP	1	CTGAACGAGTCGCACATACA	TGTTATGGACCTCCCCTGTT	20	20	50.0	50.0	58.0	57.9	13.6	0.0	0.076558	75470	76113	1316-1331	1.000000	81.6
clpP_p2	clpP	2	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	58.0	58.1	13.6	0.0	0.089790	75470	76113	1575-1590	1.000000	81.2
clpP_p3	clpP	3	CTGAACGAGTCGCACATACA	ATGGACAGAAAGCAGTCGAC	20	20	50.0	50.0	58.0	57.9	13.6	7.7	0.108866	75470	76113	1459-1474	1.000000	81.2
clpP_p4	clpP	4	ATGATCTGAACGAGTCGCAC	TGTTATGGACCTCCCCTGTT	20	20	50.0	50.0	58.1	57.9	13.6	0.0	0.139113	75470	76113	1321-1336	1.000000	81.1
clpP_p5	clpP	5	GATCTGAACGAGTCGCACAT	TGTTATGGACCTCCCCTGTT	20	20	50.0	50.0	58.1	57.9	13.6	0.0	0.139113	75470	76113	1319-1334	1.000000	81.1
petD_p1	petD	1	CCCTTGAACTCCGCAATCTT	TTCCCTAGATACGCACATCG	20	20	50.0	50.0	58.2	56.9	0.0	0.0	1.253439	80641	81896	1376-1382	1.000000	71.3
petD_p2	petD	2	TTCCCTTGAACTCCGCAATC	TTCCCTAGATACGCACATCG	20	20	50.0	50.0	58.2	56.9	0.0	0.0	1.258607	80641	81896	1378-1384	1.000000	71.3
petD_p3	petD	3	CCCTTGAACTCCGCAATCTT	GCGACTATTCCCTAGATACGC	20	21	50.0	52.4	58.2	57.9	0.0	0.0	1.307636	80641	81896	1383-1389	1.000000	70.9
petD_p4	petD	4	TTCCCTTGAACTCCGCAATC	GCGACTATTCCCTAGATACGC	20	21	50.0	52.4	58.2	57.9	0.0	0.0	1.312804	80641	81896	1385-1391	1.000000	70.8
petD_p5	petD	5	TTTCCCTTGAACTCCGCAAT	TTCCCTAGATACGCACATCG	20	20	45.0	50.0	57.7	56.9	0.0	0.0	1.366188	80641	81896	1379-1385	1.000000	70.4
rpl14-rpl16_p1	rpl14-rpl16	1	AGCATATCGGCGATTGCTAG	AGCAGCATCCAAAATGCCTA	20	20	50.0	45.0	58.0	57.9	35.6	6.4	0.150258	85276	85490	352-363	1.000000	82.4
rpl14-rpl16_p2	rpl14-rpl16	2	AGCATATCGGCGATTGCTAG	TAGCAGCATCCAAAATGCCT	20	20	50.0	45.0	58.0	57.9	35.6	6.4	0.150258	85276	85490	353-364	1.000000	82.4
rpl14-rpl16_p3	rpl14-rpl16	3	CATATCGGCGATTGCTAGCT	AGCAGCATCCAAAATGCCTA	20	20	50.0	45.0	58.0	57.9	28.9	6.4	0.150258	85276	85490	350-361	1.000000	82.4
rpl14-rpl16_p4	rpl14-rpl16	4	CATATCGGCGATTGCTAGCT	TAGCAGCATCCAAAATGCCT	20	20	50.0	45.0	58.0	57.9	28.9	6.4	0.150258	85276	85490	351-362	1.000000	82.4
rpl14-rpl16_p5	rpl14-rpl16	5	GAGCATATCGGCGATTGCTA	AGCAGCATCCAAAATGCCTA	20	20	50.0	45.0	58.0	57.9	36.0	6.4	0.152154	85276	85490	353-364	1.000000	82.3
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	GGAACAGGAGAAAGATTTCCCA	AAATGGTAGACACGCGAGAC	22	20	45.5	50.0	58.0	58.0	12.4	15.9	2.042115	98149	98868	810-823	1.000000	65.5
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TGGAACAGGAGAAAGATTTCCC	AAATGGTAGACACGCGAGAC	22	20	45.5	50.0	58.0	58.0	1.7	15.9	2.042115	98149	98868	811-824	1.000000	65.5
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	GGAACAGGAGAAAGATTTCCCA	TGAAATGGTAGACACGCGAG	22	20	45.5	50.0	58.0	58.0	12.4	15.9	2.043486	98149	98868	812-825	1.000000	65.5
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	TGGAACAGGAGAAAGATTTCCC	TGAAATGGTAGACACGCGAG	22	20	45.5	50.0	58.0	58.0	1.7	15.9	2.043486	98149	98868	813-826	1.000000	65.5
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	GGAACAGGAGAAAGATTTCCCA	GCCTTGGTGGTGAAATGGTA	22	20	45.5	50.0	58.0	58.1	12.4	0.0	2.116413	98149	98868	822-835	1.000000	64.9
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CCTCGCAATCACTAGCCAAT	AGAAGTTCCGGGAGTTACGA	20	20	50.0	50.0	58.0	58.1	0.0	1.7	0.125818	113216	113958	1437-1439	1.000000	81.8
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	TGTCCCCTCCAGTCAAGAAT	AGAAGTTCCGGGAGTTACGA	20	20	50.0	50.0	58.0	58.1	0.0	1.7	0.129652	113216	113958	1462-1464	1.000000	81.7
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CCTTGTTCCTACATGACCCG	AGAAGTTCCGGGAGTTACGA	20	20	55.0	50.0	58.0	58.1	0.0	1.7	0.109035	113216	113958	1604-1606	1.000000	81.6
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CCTTGTTCCTACATGACCCG	TGGGTTGAGAACGGGAATTG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.119889	113216	113958	1558-1560	1.000000	81.6
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CCTTGTTCCTACATGACCCG	TTGGTCATGGGTTGAGAACG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.133299	113216	113958	1565-1567	1.000000	81.5
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGGATTCGAACCTACGACCA	GCTTTTTGTTGCTGTCGGAA	20	20	50.0	45.0	58.2	57.8	15.0	0.0	0.358927	114031	115067	1204-1218	1.000000	79.4
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	AGGATTCGAACCTACGACCA	AAGGCTTTTTGTTGCTGTCG	20	20	50.0	45.0	58.2	57.8	15.0	0.0	0.364958	114031	115067	1207-1221	1.000000	79.3
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	AGGATTCGAACCTACGACCA	CGAAGGCTTTTTGTTGCTGT	20	20	50.0	45.0	58.2	57.8	15.0	0.0	0.364958	114031	115067	1209-1223	1.000000	79.3
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GGATTCGAACCTACGACCAG	GCTTTTTGTTGCTGTCGGAA	20	20	55.0	45.0	57.8	57.8	0.0	0.0	0.407602	114031	115067	1203-1217	1.000000	79.0
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGATTCGAACCTACGACCAG	AAGGCTTTTTGTTGCTGTCG	20	20	55.0	45.0	57.8	57.8	0.0	0.0	0.413633	114031	115067	1206-1220	1.000000	78.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCGGGCGTATCTCTTTTTA	GGAGAGCGGGGACTAATAGT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.057746	117630	118422	1768-1779	0.666667	66.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCGGGCGTATCTCTTTTTA	TGGAGAGCGGGGACTAATAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.059170	117630	118422	1769-1780	0.666667	66.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCGGGCGTATCTCTTTTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.063799	117630	118422	1233-1244	0.666667	66.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCGGGCGTATCTCTTTTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.065169	117630	118422	1234-1245	0.666667	66.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCGGGCGTATCTCTTTTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180246	117630	118422	1243-1254	0.666667	65.6
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	AGAAGTTCCGGGAGTTACGA	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.1	58.0	1.7	0.0	0.109035	133769	134511	1604-1606	1.000000	84.4
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	ATACGATAGCGGCTAGTCGA	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	57.9	58.0	30.2	0.0	0.114576	133769	134511	2009-2022	1.000000	84.4
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	AGAAGTTCCGGGAGTTACGA	CCTCGCAATCACTAGCCAAT	20	20	50.0	50.0	58.1	58.0	1.7	0.0	0.125818	133769	134511	1437-1439	1.000000	84.4
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	TGGGTTGAGAACGGGAATTG	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.119889	133769	134511	1558-1560	1.000000	84.3
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	AGAAGTTCCGGGAGTTACGA	TGTCCCCTCCAGTCAAGAAT	20	20	50.0	50.0	58.1	58.0	1.7	0.0	0.129652	133769	134511	1462-1464	1.000000	84.3
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	GGAACAGGAGAAAGATTTCCCA	20	22	50.0	45.5	58.0	58.0	15.9	12.4	2.042115	148859	149578	810-823	1.000000	65.5
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	AAATGGTAGACACGCGAGAC	TGGAACAGGAGAAAGATTTCCC	20	22	50.0	45.5	58.0	58.0	15.9	1.7	2.042115	148859	149578	811-824	1.000000	65.5
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	TGAAATGGTAGACACGCGAG	GGAACAGGAGAAAGATTTCCCA	20	22	50.0	45.5	58.0	58.0	15.9	12.4	2.043486	148859	149578	812-825	1.000000	65.5
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	TGGAACAGGAGAAAGATTTCCC	20	22	50.0	45.5	58.0	58.0	15.9	1.7	2.043486	148859	149578	813-826	1.000000	65.5
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	GCCTTGGTGGTGAAATGGTA	GGAACAGGAGAAAGATTTCCCA	20	22	50.0	45.5	58.1	58.0	0.0	12.4	2.116413	148859	149578	822-835	1.000000	64.9