Hotspot

ycf58

Genome · 66982-67437 · 456 bp

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Pi 0.29

MarkerSeek score 77.70

Primer available yes

Label ycf58

Feature Metadata

label_name: ycf58
feature_type: gene
region: Genome
length_bp: 456
variable_sites: 185
pi: 0.29
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf58_p1	GCCCTTTCCTCCTGAGAAAG	CGTATATCGAACAGACGTGCT	55.0	47.6	57.9	58.0	1544 bp	0.333333	44.2
ycf58_p2	AAAGCCCTTTCCTCCTGAGA	CGTATATCGAACAGACGTGCT	50.0	47.6	58.3	58.0	1547 bp	0.333333	43.2
ycf58_p3	AAGCCCTTTCCTCCTGAGAA	CGTATATCGAACAGACGTGCT	50.0	47.6	58.3	58.0	1546 bp	0.333333	43.2
ycf58_p4	AGCCCTTTCCTCCTGAGAAA	CGTATATCGAACAGACGTGCT	50.0	47.6	58.3	58.0	1545 bp	0.333333	43.2
ycf58_p5	CAAAAGCCCTTTCCTCCTGA	CGTATATCGAACAGACGTGCT	50.0	47.6	57.7	58.0	1549 bp	0.333333	42.9