Hotspot

ycf37

Genome · 6591-7094 · 504 bp

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Pi 0.282

MarkerSeek score 76.80

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 504
variable_sites: 202
pi: 0.282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	CATTCGCACCAAAAATCCGT	AAACACGGGATATAGCGCAG	45.0	50.0	57.7	58.1	762 bp	0.333333	49.5
ycf37_p2	CATTCGCACCAAAAATCCGT	TGCGCTAGAAAACAGCTTCT	45.0	45.0	57.7	57.8	1135 bp	0.333333	49.2
ycf37_p3	CATTCGCACCAAAAATCCGT	AAAACACGGGATATAGCGCA	45.0	45.0	57.7	57.7	763 bp	0.333333	48.0
ycf37_p4	TCATTCGCACCAAAAATCCG	AAACACGGGATATAGCGCAG	45.0	50.0	57.4	58.1	763 bp	0.333333	47.2
ycf37_p5	CATTCGCACCAAAAATCCGT	TGTGATGCTGTAGGATGGGT	45.0	50.0	57.7	58.4	1182 bp	0.333333	47.2