Hotspot

ycf29

Genome · 153509-154135 · 627 bp

Back to Vertebrata

Pi 0.176

MarkerSeek score 79.80

Primer available yes

Label ycf29

Feature Metadata

label_name: ycf29
feature_type: gene
region: Genome
length_bp: 627
variable_sites: 156
pi: 0.176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf29_p1	GCTTGTTCCTGTCATTTGAGC	CCAAGAACCAATATGGCGGA	47.6	50.0	58.1	57.9	1311 bp	0.333333	44.4
ycf29_p2	GCTTGTTCCTGTCATTTGAGC	TTGTGACTCCATCATACGCG	47.6	50.0	58.1	58.1	1265 bp	0.333333	44.2
ycf29_p3	GCTTGTTCCTGTCATTTGAGC	CAAGAGGTTACGGCAATGGA	47.6	50.0	58.1	57.9	1285 bp	0.333333	44.0
ycf29_p4	GCTTGTTCCTGTCATTTGAGC	GGCAATGGATTGTGACTCCA	47.6	50.0	58.1	58.2	1274 bp	0.333333	43.5
ycf29_p5	GCTTGTTCCTGTCATTTGAGC	ACCAATATGGCGGATGTAGC	47.6	50.0	58.1	57.8	1305 bp	0.333333	43.4