Hotspot

ycf23

Genome · 45757-46596 · 840 bp

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Pi 0.217

MarkerSeek score 79.30

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 840
variable_sites: 257
pi: 0.217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	CAAATTGCTTCCGCTCCAAG	CCCTCTCTCAAACCATAAGCA	50.0	47.6	58.0	57.4	1289 bp	0.333333	40.2
ycf23_p2	CAAATTGCTTCCGCTCCAAG	GATGTTCCCTCTCTCAAACCA	50.0	47.6	58.0	57.3	1295 bp	0.333333	39.6
ycf23_p3	AAATTGCTTCCGCTCCAAGA	CCCTCTCTCAAACCATAAGCA	45.0	47.6	58.1	57.4	1288 bp	0.333333	39.5
ycf23_p4	AATTGCTTCCGCTCCAAGAA	CCCTCTCTCAAACCATAAGCA	45.0	47.6	58.1	57.4	1287 bp	0.333333	39.5
ycf23_p5	GAAACTGCATGCCCTACTGA	CCCTCTCTCAAACCATAAGCA	50.0	47.6	57.9	57.4	1238 bp	0.333333	39.3