Hotspot

moeB

Genome · 148547-149617 · 1071 bp

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Pi 0.228

MarkerSeek score 79.60

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1071
variable_sites: 348
pi: 0.228
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	TCCTATTAGCGTTTCTTCTGCT	GGACTTGAACCCTCGACATC	40.9	55.0	57.3	58.0	1850-1857 bp	0.666667	53.0
moeB_p2	TCCTATTAGCGTTTCTTCTGCT	CTTGAACCCTCGACATCAGG	40.9	55.0	57.3	58.0	1847-1854 bp	0.666667	52.9
moeB_p3	TCCTATTAGCGTTTCTTCTGCT	GAAGGACTTGAACCCTCGAC	40.9	55.0	57.3	57.9	1853-1860 bp	0.666667	52.4
moeB_p4	TCCTATTAGCGTTTCTTCTGCT	TACCCATACTGCGAGCTGTA	40.9	50.0	57.3	57.9	2391 bp	0.333333	30.9
moeB_p5	TCCTATTAGCGTTTCTTCTGCT	GGAAACCTGCGTTCTACCAA	40.9	50.0	57.3	58.1	1830 bp	0.333333	30.5