Hotspot

dnaB

Genome · 146149-147942 · 1794 bp

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Pi 0.212

MarkerSeek score 78.10

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1794
variable_sites: 544
pi: 0.212
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTCCACTGCTCTACCAA	TGAACTTCATACAAACCTAGCA	50.0	36.4	58.0	55.4	2079 bp	0.333333	16.4
dnaB_p2	TCCACTGCTCTACCAACTGA	TGAACTTCATACAAACCTAGCA	50.0	36.4	58.0	55.4	2075 bp	0.333333	16.4
dnaB_p3	TTCAGTCCACTGCTCTACCA	TGAACTTCATACAAACCTAGCA	50.0	36.4	58.0	55.4	2080 bp	0.333333	16.4
dnaB_p4	AGTCCACTGCTCTACCAACT	TGAACTTCATACAAACCTAGCA	50.0	36.4	58.0	55.4	2077 bp	0.333333	16.3
dnaB_p5	TTTGAACTGGTGACACGAGG	TGAACTTCATACAAACCTAGCA	50.0	36.4	58.1	55.4	2103 bp	0.333333	16.0