Hotspot

carA

Genome · 12944-14122 · 1179 bp

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Pi 0.198

MarkerSeek score 81.80

Primer available yes

Label carA

Feature Metadata

label_name: carA
feature_type: gene
region: Genome
length_bp: 1179
variable_sites: 327
pi: 0.198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA_p1	TTATGGTGCGAATGGGATGC	ACAACAGAAGCACCAGAAGG	50.0	50.0	58.7	58.0	1476 bp	0.333333	47.7
carA_p2	TTATGGTGCGAATGGGATGC	GGACAACAGAAGCACCAGAA	50.0	50.0	58.7	58.0	1478 bp	0.333333	47.6
carA_p3	AACTTATGGTGCGAATGGGA	ACAACAGAAGCACCAGAAGG	45.0	50.0	57.2	58.0	1479 bp	0.333333	46.7
carA_p4	AACTTATGGTGCGAATGGGA	GGACAACAGAAGCACCAGAA	45.0	50.0	57.2	58.0	1481 bp	0.333333	46.7
carA_p5	TTATGGTGCGAATGGGATGC	GCACCAGAAGGACACTTACC	50.0	55.0	58.7	58.2	1467 bp	0.333333	46.4