Hotspot

argB

Genome · 139722-140582 · 861 bp

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Pi 0.196

MarkerSeek score 79.50

Primer available yes

Label argB

Feature Metadata

label_name: argB
feature_type: gene
region: Genome
length_bp: 861
variable_sites: 242
pi: 0.196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
argB_p1	GAGACTGACGGGATTCGAAC	TAGCACCACCAGGATAACCA	55.0	50.0	58.1	58.0	1635-1656 bp	1.0	88.1
argB_p2	CGACCTTGGGCTTATGAGTC	TAGCACCACCAGGATAACCA	55.0	50.0	58.1	58.0	1383-1389 bp	1.0	87.4
argB_p3	GCTAGCAACGACCTACAACA	TAGCACCACCAGGATAACCA	50.0	50.0	57.9	58.0	2021-2026 bp	0.666667	73.8
argB_p4	CGACCTTGGGCTTATGAGTC	GGAAGAATAGCACCACCAGG	55.0	55.0	58.1	58.0	1390 bp	0.333333	52.5
argB_p5	GCTAGCAACGACCTACAACA	GGAAGAATAGCACCACCAGG	50.0	55.0	57.9	58.0	2028 bp	0.333333	52.4