Hotspot

accB

Genome · 109971-110483 · 513 bp

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Pi 0.196

MarkerSeek score 80.30

Primer available yes

Label accB

Feature Metadata

label_name: accB
feature_type: gene
region: Genome
length_bp: 513
variable_sites: 139
pi: 0.196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accB_p1	ACGGCCAACACGAAAAGTTA	ACCATTCTAGTTTTGGCGCA	45.0	45.0	58.3	58.1	1907 bp	0.333333	49.9
accB_p2	AGGAAAACGTGCTTCAGGTC	ACCATTCTAGTTTTGGCGCA	50.0	45.0	58.4	58.1	1727 bp	0.333333	49.3
accB_p3	GGAAAACGTGCTTCAGGTCT	ACCATTCTAGTTTTGGCGCA	50.0	45.0	58.4	58.1	1726 bp	0.333333	49.3
accB_p4	ACCAAATATAGCACGGCCAA	ACCATTCTAGTTTTGGCGCA	45.0	45.0	57.6	58.1	1919 bp	0.333333	49.1
accB_p5	ACGGCCAACACGAAAAGTTA	TGTCCAGGTTTTGCCCATTT	45.0	45.0	58.3	58.2	1437 bp	0.333333	49.1