Hotspot

ConsOrf5-trnM

Genome · 65369-65898 · 530 bp

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Pi 0.126

MarkerSeek score 79.50

Primer available yes

Label ConsOrf5-trnM

Feature Metadata

label_name: ConsOrf5-trnM
feature_type: igs
region: Genome
length_bp: 530
variable_sites: 95
pi: 0.126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf5-trnM_p1	ACTCCAACAAAAAGTTTTCCAAGA	ACTCTCCTCATGGTTGGGAA	33.3	50.0	57.6	58.0	898 bp	0.333333	18.1
ConsOrf5-trnM_p2	ACTCCAACAAAAAGTTTTCCAAGA	CTTTCTCAGGAGGAAAGGGC	33.3	55.0	57.6	57.9	1244 bp	0.333333	17.5
ConsOrf5-trnM_p3	ACTCCAACAAAAAGTTTTCCAAGA	TATCCCCTTCAGCAGTAGCA	33.3	50.0	57.6	57.8	745 bp	0.333333	17.1
ConsOrf5-trnM_p4	ACTCCAACAAAAAGTTTTCCAAGA	CCCTTCAGCAGTAGCAACTT	33.3	50.0	57.6	57.8	741 bp	0.333333	17.0
ConsOrf5-trnM_p5	ACTCCAACAAAAAGTTTTCCAAGA	TCTCAGGAGGAAAGGGCTTT	33.3	50.0	57.6	58.3	1241 bp	0.333333	16.5