Hotspot

trnR-trnV

Genome · 29114-29148 · 35 bp

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Pi 0.304

MarkerSeek score 79.70

Primer available yes

Label trnR-trnV

Feature Metadata

label_name: trnR-trnV
feature_type: igs
region: Genome
length_bp: 35
variable_sites: 13
pi: 0.304
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnV_p1	AGAAGGTCCCTGTCCTGATC	CTGGTTCAAGTCCAGTACCG	55.0	55.0	58.1	57.9	83-96 bp	1.0	90.3
trnR-trnV_p2	AGAAGGTCCCTGTCCTGATC	TTACAAGCAGGTGGTCACTG	55.0	50.0	58.1	57.7	100-113 bp	1.0	88.9
trnR-trnV_p3	GTCCCTGTCCTGATCCCTTA	TTACAAGCAGGTGGTCACTG	55.0	50.0	57.8	57.7	95-108 bp	1.0	88.6
trnR-trnV_p4	AGAAGGTCCCTGTCCTGATC	CAGGTGGTCACTGGTTCAAG	55.0	55.0	58.1	58.4	93-106 bp	1.0	87.8
trnR-trnV_p5	GTCCCTGTCCTGATCCCTTA	CTGGTTCAAGTCCAGTACCG	55.0	55.0	57.8	57.9	90-91 bp	0.666667	73.5