Hotspot

ConsOrf5

Genome · 64277-65368 · 1092 bp

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Pi 0.269

MarkerSeek score 80.30

Primer available yes

Label ConsOrf5

Feature Metadata

label_name: ConsOrf5
feature_type: gene
region: Genome
length_bp: 1092
variable_sites: 413
pi: 0.269
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf5_p1	CGATGGGATGGCGGATTATT	TGCGATCCGTTTTTAGGTGT	50.0	45.0	57.9	57.8	1773-1794 bp	1.0	87.9
ConsOrf5_p2	CGATGGGATGGCGGATTATT	TGTGCGATCCGTTTTTAGGT	50.0	45.0	57.9	57.8	1775-1796 bp	1.0	87.9
ConsOrf5_p3	CGATGGGATGGCGGATTATT	ACTCATGTGCGATCCGTTTT	50.0	45.0	57.9	58.2	1780-1801 bp	1.0	87.7
ConsOrf5_p4	CGATGGGATGGCGGATTATT	TATCCCCTTCAGCAGTAGCA	50.0	50.0	57.9	57.8	1908 bp	0.333333	51.0
ConsOrf5_p5	CGATGGGATGGCGGATTATT	CCCTTCAGCAGTAGCAACTT	50.0	50.0	57.9	57.8	1904 bp	0.333333	50.9