Hotspot

ccsA-ndhD

SSC · 114387-114603 · 217 bp

Back to Veronicastrum

Pi 0.0298

MarkerSeek score 67.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 18
pi: 0.0298
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCAGCAATTGTGGCGTCTA	AGGTATTGGTATGTACCCCGA	45.0	47.6	57.8	57.7	417-423 bp	1.0	71.7
ccsA-ndhD_p2	GCAATTGTGGCGTCTATTGG	AGGTATTGGTATGTACCCCGA	50.0	47.6	57.8	57.7	413-419 bp	1.0	71.6
ccsA-ndhD_p3	AATTCAGCAATTGTGGCGTC	AGGTATTGGTATGTACCCCGA	45.0	47.6	57.6	57.7	419-425 bp	1.0	70.3
ccsA-ndhD_p4	TCAGCAATTGTGGCGTCTAT	AGGTATTGGTATGTACCCCGA	45.0	47.6	57.6	57.7	416-422 bp	1.0	70.0
ccsA-ndhD_p5	ATTCAGCAATTGTGGCGTCT	AGGTATTGGTATGTACCCCGA	45.0	47.6	58.5	57.7	418-424 bp	1.0	69.5