Hotspot

trnL-UAG-ccsA

SSC · 113243-113414 · 172 bp

Back to Veronicastrum

Pi 0.0377

MarkerSeek score 66.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 172
variable_sites: 20
pi: 0.0377
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	57.9	53.2	218-258 bp	1.0	50.6
trnL-UAG-ccsA_p2	GGTAGACACGCTGCTCTTAG	TGCTCTAAGGTTGAAAATATCA	55.0	31.8	57.8	53.2	220-260 bp	1.0	50.5
trnL-UAG-ccsA_p3	GTAGACACGCTGCTCTTAGG	TGCTCTAAGGTTGAAAATATCA	55.0	31.8	57.8	53.2	219-259 bp	1.0	50.5
trnL-UAG-ccsA_p4	GTGAAATCGGTAGACACGCT	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	58.0	53.2	228-268 bp	1.0	50.3
trnL-UAG-ccsA_p5	TGAAATCGGTAGACACGCTG	TGCTCTAAGGTTGAAAATATCA	50.0	31.8	58.0	53.2	227-267 bp	1.0	50.3