Hotspot

rps8-rpl14

LSC · 79990-80201 · 212 bp

Back to Veronicastrum

Pi 0.0131

MarkerSeek score 62.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 10
pi: 0.0131
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATGCTATGCGAACAACTCT	ATCCCCCGAGAATTGAGACA	42.9	50.0	57.5	58.1	339-362 bp	1.0	73.5
rps8-rpl14_p2	AGATGCTATGCGAACAACTCT	TCCCCCGAGAATTGAGACAA	42.9	50.0	57.5	58.3	338-361 bp	1.0	71.7
rps8-rpl14_p3	AGATGCTATGCGAACAACTC	ATCCCCCGAGAATTGAGACA	45.0	50.0	55.9	58.1	339-362 bp	1.0	69.3
rps8-rpl14_p4	GATGCTATGCGAACAACTCT	ATCCCCCGAGAATTGAGACA	45.0	50.0	55.9	58.1	338-361 bp	1.0	69.3
rps8-rpl14_p5	AGATGCTATGCGAACAACTCT	GCAATCCCCCGAGAATTGAG	42.9	55.0	57.5	58.7	342-365 bp	1.0	68.9