Hotspot

petA-psbJ

LSC · 62972-63936 · 965 bp

Back to Veronicastrum

Pi 0.0115

MarkerSeek score 70.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 41
pi: 0.0115
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1018-1035 bp	1.0	79.0
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1020-1037 bp	1.0	78.9
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1017-1034 bp	1.0	78.5
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1019-1036 bp	1.0	78.5
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1017-1034 bp	1.0	78.1