Hotspot

ycf4-cemA

LSC · 60461-61150 · 690 bp

Back to Veronicastrum

Pi 0.00717

MarkerSeek score 65.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 16
pi: 0.00717
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGACCCGTACTGATGAG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.1	58.1	860-899 bp	1.0	84.2
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	790-829 bp	1.0	84.0
ycf4-cemA_p3	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	819-858 bp	1.0	83.1
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.7	58.1	793-832 bp	1.0	82.4
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.4	58.1	797-836 bp	1.0	82.1