Hotspot

accD-psaI

LSC · 58570-59355 · 786 bp

Back to Veronicastrum

Pi 0.00652

MarkerSeek score 69.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 22
pi: 0.00652
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	949-989 bp	1.0	83.9
accD-psaI_p2	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	883-923 bp	1.0	83.8
accD-psaI_p3	CTCCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	879-919 bp	1.0	83.8
accD-psaI_p4	CCAGAAGGGCTTATTCGACC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	955-995 bp	1.0	83.3
accD-psaI_p5	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	889-929 bp	1.0	83.2