Hotspot

atpH-atpI

LSC · 13373-14363 · 991 bp

Back to Veronicastrum

Pi 0.00738

MarkerSeek score 67.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 991
variable_sites: 23
pi: 0.00738
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1124-1150 bp	1.0	80.3
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1141-1167 bp	1.0	79.3
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.3	58.3	1136-1162 bp	1.0	79.0
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1076-1102 bp	1.0	78.3
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	57.6	58.3	1137-1163 bp	1.0	77.7