Hotspot

trnS-GCU-trnG-UCC

LSC · 8167-8885 · 719 bp

Back to Veronicastrum

Pi 0.018

MarkerSeek score 69.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 47
pi: 0.018
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCTTTAACA	50.0	40.0	58.0	54.8	823-847 bp	1.0	60.2
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	ACCGAAAGACCCTTTAACAA	50.0	40.0	58.0	54.8	822-846 bp	1.0	60.2
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCTTTAACA	50.0	40.0	58.0	54.8	822-846 bp	1.0	60.2
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ACCGAAAGACCCTTTAACAA	50.0	40.0	58.0	54.8	821-845 bp	1.0	60.2
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACCGAAAGACCCTTTAACAA	50.0	40.0	58.2	54.8	876-900 bp	1.0	58.7