Hotspot

trnK-UUU-rps16

LSC · 4247-5111 · 865 bp

Back to Veronicastrum

Pi 0.0103
MarkerSeek score 65.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
865
variable_sites
30
pi
0.0103
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 654-1018 bp 1.0 85.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 653-1017 bp 1.0 85.0
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 668-1032 bp 1.0 84.2
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 663-1027 bp 1.0 84.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 657-1021 bp 1.0 83.4