Hotspot

ndhF-rpl32

SSC · 109502-110156 · 655 bp

Back to Veronica

Pi 0.086

MarkerSeek score 78.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 655
variable_sites: 202
pi: 0.086
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGTATGATCCACGC	GACTCCCCACCTTGGAAATG	50.0	55.0	58.1	58.2	951-1116 bp	0.92	87.0
ndhF-rpl32_p2	ACGAAAGGTATGATCCACGC	AGAAAACGCTTTTAACGCCG	50.0	45.0	58.1	58.0	659-822 bp	0.8	83.3
ndhF-rpl32_p3	CGAAAGGTATGATCCACGCA	AGAAAACGCTTTTAACGCCG	50.0	45.0	58.1	58.0	658-821 bp	0.8	83.3
ndhF-rpl32_p4	ACGAAAGGTATGATCCACGC	CGCTTGATCCCCCTTTCTTT	50.0	50.0	58.1	58.2	725-790 bp	0.2	56.9
ndhF-rpl32_p5	CGAAAGGTATGATCCACGCA	CGCTTGATCCCCCTTTCTTT	50.0	50.0	58.1	58.2	724-789 bp	0.2	56.9