Hotspot

psbE-petL

LSC · 63449-63989 · 541 bp

Back to Veronica

Pi 0.058

MarkerSeek score 80.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 121
pi: 0.058
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TCGTTCGGGTTTGATTGTGT	AAGCGCTCTTAGTTCAGCTC	45.0	50.0	58.0	58.0	1561-1887 bp	0.92	88.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	57.9	58.0	1233-1559 bp	0.92	88.4
psbE-petL_p3	ATGGAATTCCTTGTCGGCTC	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.0	58.0	1335-1648 bp	0.84	85.2
psbE-petL_p4	GGTACAGCTAGGCCATGAAC	AAGCGCTCTTAGTTCAGCTC	55.0	50.0	58.0	58.0	1463-1789 bp	0.8	83.6
psbE-petL_p5	TCCTTGTCGGCTCTCTGTAA	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	58.1	58.0	1328 bp	0.12	44.2