Hotspot

ycf4-cemA

LSC · 59263-59924 · 662 bp

Back to Veronica

Pi 0.0825

MarkerSeek score 77.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 184
pi: 0.0825
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGGGGATTTCCTGGAAA	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.1	58.0	1326-1517 bp	1.0	90.6
ycf4-cemA_p2	GACCCGTACTGATGCGAATT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.1	58.0	1180-1371 bp	0.88	85.8
ycf4-cemA_p3	CGTCGCATATTCCTCCGATT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	57.9	58.0	1302-1493 bp	0.6	74.6
ycf4-cemA_p4	AAACCGTCGCATATTCCTCC	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	955-1146 bp	0.56	72.8
ycf4-cemA_p5	AAACCGTCGCATATTCCTCC	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.1	58.0	1306-1497 bp	0.56	72.7