Hotspot

ndhC-trnV-UAC

LSC · 49365-50308 · 944 bp

Back to Veronica

Pi 0.0752

MarkerSeek score 81.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 275
pi: 0.0752
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGATCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	28.0	50.0	55.9	57.8	989-1209 bp	1.0	52.0
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	27.3	50.0	52.7	57.8	986-1206 bp	1.0	52.0
ndhC-trnV-UAC_p3	GATCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	29.2	50.0	54.6	57.8	988-1208 bp	1.0	52.0
ndhC-trnV-UAC_p4	AGATCATATTCGTAAAGCAAAAACA	GAAGGTCTACGGTTCGAGTC	28.0	55.0	55.9	57.5	988-1208 bp	1.0	52.0
ndhC-trnV-UAC_p5	AAGATCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	26.9	50.0	56.5	57.8	990-1210 bp	1.0	52.0