Hotspot

trnF-GAA-ndhJ

LSC · 47144-47700 · 557 bp

Back to Veronica

Pi 0.0604

MarkerSeek score 79.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 128
pi: 0.0604
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAATTACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	586-619 bp	1.0	52.0
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	TGAATTACAAGATGCTCATTGA	50.0	31.8	57.5	53.8	589-622 bp	1.0	52.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGAATTACAAGATGCTCATTGA	50.0	30.4	58.1	54.4	587-620 bp	1.0	52.0
trnF-GAA-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	TGAATTACAAGATGCTCATTGA	50.0	31.8	57.4	53.8	609-642 bp	1.0	52.0
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGAATTACAAGATGCTCATTG	50.0	33.3	58.1	52.2	586-619 bp	1.0	52.0