Hotspot

ycf3-trnS-GGA

LSC · 43375-44223 · 849 bp

Back to Veronica

Pi 0.0623

MarkerSeek score 81.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 174
pi: 0.0623
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGCGATCTAGACA	CCCTCGGTAAACAAAAGCCT	45.5	50.0	57.8	58.1	405-965 bp	1.0	73.8
ycf3-trnS-GGA_p2	CCATTTATCCGCGATCTAGACA	TTCCAATGCTACGCCTTGAA	45.5	45.0	57.8	57.8	376-936 bp	1.0	73.1
ycf3-trnS-GGA_p3	TCCATTTATCCGCGATCTAGAC	CCCTCGGTAAACAAAAGCCT	45.5	50.0	57.6	58.1	406-966 bp	1.0	71.8
ycf3-trnS-GGA_p4	CCATTTATCCGCGATCTAGACA	ACCCTCGGTAAACAAAAGCC	45.5	50.0	57.8	58.4	406-966 bp	1.0	71.5
ycf3-trnS-GGA_p5	TCCATTTATCCGCGATCTAGAC	TTCCAATGCTACGCCTTGAA	45.5	45.0	57.6	57.8	377-937 bp	1.0	71.0