Hotspot

psaA-ycf3

LSC · 40604-41423 · 820 bp

Back to Veronica

Pi 0.0626

MarkerSeek score 73.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 165
pi: 0.0626
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTTACTTCTGGCTCCGGC	TGAAGATTACGAGACGGTTCG	50.0	47.6	58.5	57.9	751-880 bp	1.0	79.2
psaA-ycf3_p2	TTTTTACTTCTGGCTCCGGC	TGGTTGAAGATTACGAGACGG	50.0	47.6	58.5	57.8	755-884 bp	1.0	78.4
psaA-ycf3_p3	TTTTTACTTCTGGCTCCGGC	GGTTGAAGATTACGAGACGGT	50.0	47.6	58.5	57.8	754-883 bp	1.0	78.4
psaA-ycf3_p4	TTGGCCCATTCTTCGAAAGA	TGAAGATTACGAGACGGTTCG	45.0	47.6	57.4	57.9	800-929 bp	1.0	78.4
psaA-ycf3_p5	TTGGCCCATTCTTCGAAAGA	TGGTTGAAGATTACGAGACGG	45.0	47.6	57.4	57.8	804-933 bp	1.0	77.6