Hotspot

rps16-trnQ-UUG

LSC · 5997-6561 · 565 bp

Back to Veronica

Pi 0.0769

MarkerSeek score 72.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 153
pi: 0.0769
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AATGGTGGGTGGGAAAATCC	GGAAATGCGGGTAAGCCTAA	50.0	50.0	58.0	58.0	1313-1466 bp	0.8	83.2
rps16-trnQ-UUG_p2	TTTCGACTCGAGCTCCATTG	GGAAATGCGGGTAAGCCTAA	50.0	50.0	58.0	58.0	1577 bp	0.12	44.4
rps16-trnQ-UUG_p3	TTTCGACTCGAGCTCCATTG	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1410 bp	0.12	44.3
rps16-trnQ-UUG_p4	TTTCGACTCGAGCTCCATTG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1415 bp	0.12	44.3
rps16-trnQ-UUG_p5	TTTCGACTCGAGCTCCATTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	862 bp	0.12	44.2