Hotspot

rpl32-trnL-UAG

SSC · 112202-112880 · 679 bp

Back to Verbesina

Pi 0.0385
MarkerSeek score 70.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
679
variable_sites
25
pi
0.0385
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGGAAAGGATATGGGGCG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 790-812 bp 1.0 82.5
rpl32-trnL-UAG_p2 AAAGGAAAGGATATGGGGCG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 791-813 bp 1.0 82.5
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 772-794 bp 1.0 82.4
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 773-795 bp 1.0 82.4
rpl32-trnL-UAG_p5 AAAGGAAAGGATATGGGGCG GAAGTAGCAAAGCCTTGGGT 50.0 50.0 57.6 58.1 1291-1336 bp 1.0 81.3