Hotspot

rps18-rpl20

LSC · 67989-68236 · 248 bp

Back to Verbesina

Pi 0.0165

MarkerSeek score 63.90

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 4
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AACCGAGTCGACCACTAGAA	AGACAGTTGCTTCTTAATCGT	50.0	38.1	58.1	55.3	412-417 bp	1.0	51.1
rps18-rpl20_p2	AAGAACCGAGTCGACCACTA	AGACAGTTGCTTCTTAATCGT	50.0	38.1	58.1	55.3	415-420 bp	1.0	51.1
rps18-rpl20_p3	TTGAAAGAACCGAGTCGACC	AGACAGTTGCTTCTTAATCGT	50.0	38.1	57.9	55.3	419-424 bp	1.0	50.7
rps18-rpl20_p4	AGAACCGAGTCGACCACTAG	AGACAGTTGCTTCTTAATCGT	55.0	38.1	58.5	55.3	414-419 bp	1.0	47.5
rps18-rpl20_p5	CCGAGTCGACCACTAGAACT	AGACAGTTGCTTCTTAATCGT	55.0	38.1	58.5	55.3	410-415 bp	1.0	47.5