Hotspot

pafI-trnS-GCU

LSC · 44286-45270 · 985 bp

Back to Verbesina

Pi 0.0124

MarkerSeek score 61.50

Primer available yes

Label pafI-trnS-GCU

Feature Metadata

label_name: pafI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 11
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GCU_p1	TTTCCGTTTATCCGCGATCT	CAACCACTCAGCCATCTCTC	45.0	55.0	57.4	58.0	936-1032 bp	1.0	80.0
pafI-trnS-GCU_p2	TTTCCGTTTATCCGCGATCT	ATGCTACGCTTTCAACCACT	45.0	45.0	57.4	57.8	948-1044 bp	1.0	78.7
pafI-trnS-GCU_p3	TTTCCGTTTATCCGCGATCT	GGATTCGAACCCTCGGTAAG	45.0	55.0	57.4	57.8	991-1087 bp	1.0	78.4
pafI-trnS-GCU_p4	TTTCCGTTTATCCGCGATCT	CCAATGCTACGCTTTCAACC	45.0	50.0	57.4	57.7	951-1047 bp	1.0	78.1
pafI-trnS-GCU_p5	TTTCCGTTTATCCGCGATCT	TCAACCACTCAGCCATCTCT	45.0	50.0	57.4	58.4	937-1033 bp	1.0	77.4