Hotspot

trnS-UGA-psbZ

LSC · 35359-35696 · 338 bp

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Pi 0.0178

MarkerSeek score 63.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 338
variable_sites: 6
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	457-458 bp	1.0	79.4
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	459-460 bp	1.0	79.0
trnS-UGA-psbZ_p3	TCAACCACTCAGCCATCTCT	CCAACCATCAGGAGAAGCAA	50.0	50.0	58.4	57.8	458-459 bp	1.0	76.8
trnS-UGA-psbZ_p4	TCAACCACTCAGCCATCTCT	GACCAACCATCAGGAGAAGC	50.0	55.0	58.4	58.3	460-461 bp	1.0	76.4
trnS-UGA-psbZ_p5	TTCAACCACTCAGCCATCTC	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.5	57.8	459-460 bp	1.0	75.7