Hotspot

trnE-UUC-rpoB

LSC · 12271-13188 · 918 bp

Back to Verbesina

Pi 0.0177

MarkerSeek score 57.50

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 918
variable_sites: 16
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	TAACCACTGGACCATAGGGG	TGATTAAATCCAGGTATTGTGG	55.0	36.4	58.1	53.9	955-965 bp	1.0	42.7
trnE-UUC-rpoB_p2	GGGACTTGAACCCTCGTTTT	TGATTAAATCCAGGTATTGTGG	50.0	36.4	58.0	53.9	996-1006 bp	1.0	42.6
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	TGATTAAATCCAGGTATTGTGG	50.0	36.4	58.1	53.9	986-996 bp	1.0	42.6
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	TGATTAAATCCAGGTATTGTGG	50.0	36.4	58.1	53.9	991-1001 bp	1.0	42.6
trnE-UUC-rpoB_p5	TCTCCGTGAAAGAGAGAGGT	TGATTAAATCCAGGTATTGTGG	50.0	36.4	57.4	53.9	977-987 bp	1.0	42.6