Hotspot

psbI-trnS-GCU

LSC · 8503-8640 · 138 bp

Back to Verbesina

Pi 0.0217

MarkerSeek score 65.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 3
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	273 bp	1.0	69.4
psbI-trnS-GCU_p2	CCTATCCAATGATCCAGGACG	AGTTAGTCGTACCGAGGGTT	52.4	50.0	57.7	57.8	219 bp	1.0	68.8
psbI-trnS-GCU_p3	CCTATCCAATGATCCAGGACG	GAGTTAGTCGTACCGAGGGT	52.4	55.0	57.7	58.3	220 bp	1.0	68.4
psbI-trnS-GCU_p4	CCTATCCAATGATCCAGGACG	GTTAGTCGTACCGAGGGTTC	52.4	55.0	57.7	57.4	218 bp	1.0	66.1
psbI-trnS-GCU_p5	CCTATCCAATGATCCAGGACG	GATGGCTGAGTGGACGAAAG	52.4	55.0	57.7	58.6	264 bp	1.0	65.2