Hotspot

ycf1

SSC · 123216-128891 · 5676 bp

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Pi 0.00513

MarkerSeek score 55.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5676
variable_sites: 29
pi: 0.00513
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTCGATTGTTTCCTCCGG	CCAGACCCCTCAGAACAAAA	50.0	50.0	57.9	57.3	275-293 bp	1.0	78.8
ycf1_p2	TGTTTCCTCCGGCTTTTTCT	CCAGACCCCTCAGAACAAAA	45.0	50.0	57.6	57.3	267-285 bp	1.0	76.7
ycf1_p3	AGTTCGATTGTTTCCTCCGG	CCAGACCCCTCAGAACAAAAA	50.0	47.6	57.9	58.1	275-293 bp	1.0	75.6
ycf1_p4	AGTTCGATTGTTTCCTCCGG	CCCAGACCCCTCAGAACAAA	50.0	55.0	57.9	59.2	276-294 bp	1.0	74.3
ycf1_p5	TGTTTCCTCCGGCTTTTTCT	CCAGACCCCTCAGAACAAAAA	45.0	47.6	57.6	58.1	267-285 bp	1.0	73.6