Hotspot

rps15

SSC · 122584-122847 · 264 bp

Back to Verbena

Pi 0.00379

MarkerSeek score 40.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 1
pi: 0.00379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TCCCAACGCGTTACATAAGG	TCCAGCATTTTGGAACGGAA	50.0	45.0	58.0	58.0	1164-1172 bp	1.0	81.0
rps15_p2	CCGAGTCATTCTTCCATGCA	TCCAGCATTTTGGAACGGAA	50.0	45.0	58.0	58.0	943-951 bp	1.0	81.0
rps15_p3	GGTCTGTGCCCCAATATCTG	TCCAGCATTTTGGAACGGAA	55.0	45.0	58.0	58.0	1339-1347 bp	1.0	80.7
rps15_p4	ATCAGTCGAAGAACACCGTG	TCCAGCATTTTGGAACGGAA	50.0	45.0	57.9	58.0	1038-1046 bp	1.0	80.6
rps15_p5	CCTATGCCCTCAACTCGTTC	TCCAGCATTTTGGAACGGAA	55.0	45.0	58.1	58.0	1572-1580 bp	1.0	80.4