Hotspot

rpoA

LSC · 78299-79294 · 996 bp

Back to Verbena

Pi 0.00301

MarkerSeek score 60.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 996
variable_sites: 3
pi: 0.00301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGTGCTCCAGATTCAAAAACT	AATGGCTGTAGACCTCCGAA	38.1	50.0	56.0	58.4	1183 bp	1.0	52.6
rpoA_p2	TGTGCTCCAGATTCAAAAACT	ATGGCTGTAGACCTCCGAAA	38.1	50.0	56.0	58.4	1182 bp	1.0	52.6
rpoA_p3	TGTGCTCCAGATTCAAAAACT	TAATGGCTGTAGACCTCCGA	38.1	50.0	56.0	57.6	1184 bp	1.0	52.5
rpoA_p4	TGAATTGTGCTCCAGATTCA	AATGGCTGTAGACCTCCGAA	40.0	50.0	55.0	58.4	1188 bp	1.0	52.4
rpoA_p5	TGAATTGTGCTCCAGATTCA	ATGGCTGTAGACCTCCGAAA	40.0	50.0	55.0	58.4	1187 bp	1.0	52.4