Hotspot

petD

LSC · 76881-78096 · 1216 bp

Back to Verbena

Pi 0.00247

MarkerSeek score 60.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1216
variable_sites: 3
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGAGTTGAAGGGAATTCTCCG	TCCCTAGATACACACGTCGT	52.4	50.0	58.4	57.6	1294 bp	1.0	66.0
petD_p2	GAGTTGAAGGGAATTCTCCGA	TCCCTAGATACACACGTCGT	47.6	50.0	57.4	57.6	1293 bp	1.0	64.0
petD_p3	TGGAGTTGAAGGGAATTCTCC	TCCCTAGATACACACGTCGT	47.6	50.0	57.3	57.6	1295 bp	1.0	63.2
petD_p4	AGTTGAAGGGAATTCTCCGA	TCCCTAGATACACACGTCGT	45.0	50.0	56.2	57.6	1292 bp	1.0	62.2
petD_p5	TGAAGGGAATTCTCCGAAGAG	TCCCTAGATACACACGTCGT	47.6	50.0	57.1	57.6	1289 bp	1.0	61.8