Hotspot

matK

LSC · 1944-3470 · 1527 bp

Back to Verbena

Pi 0.00589

MarkerSeek score 62.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 9
pi: 0.00589
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGTATCTCTTCGTCCATTTGA	CCTGTCTCCGCGGTATCTAT	38.1	55.0	54.4	58.5	1689 bp	1.0	40.4
matK_p2	TGTATCTCTTCGTCCATTTGA	TTTACCTGTCTCCGCGGTAT	38.1	50.0	54.4	58.5	1693 bp	1.0	40.4
matK_p3	TCAGAATAGTGGAAATCCCA	CCTGTCTCCGCGGTATCTAT	40.0	55.0	53.3	58.5	1718 bp	1.0	40.4
matK_p4	TCAGAATAGTGGAAATCCCA	TTTACCTGTCTCCGCGGTAT	40.0	50.0	53.3	58.5	1722 bp	1.0	40.4
matK_p5	TGTATCTCTTCGTCCATTTGA	TAAGTTTACCTGTCTCCGCG	38.1	50.0	54.4	57.4	1697 bp	1.0	40.4