Hotspot

ccsA

SSC · 113952-114923 · 972 bp

Back to Verbena

Pi 0.00412

MarkerSeek score 46.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 4
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGGCGGCATATCATCTCCTA	CGTAAGAACCCGTTTTGGAA	50.0	45.0	58.1	56.3	1107-1108 bp	1.0	66.3
ccsA_p2	GAGTGGCGGCATATCATCTC	CGTAAGAACCCGTTTTGGAA	55.0	45.0	58.3	56.3	1110-1111 bp	1.0	64.5
ccsA_p3	TGGCGGCATATCATCTCCTA	CGTAAGAACCCGTTTTGGAAT	50.0	42.9	58.1	56.8	1107-1108 bp	1.0	62.5
ccsA_p4	GAGTGGCGGCATATCATCTC	CGTAAGAACCCGTTTTGGAAT	55.0	42.9	58.3	56.8	1110-1111 bp	1.0	60.7
ccsA_p5	TGGCGGCATATCATCTCCTA	CGTAAGAACCCGTTTTGGAATT	50.0	40.9	58.1	57.5	1107-1108 bp	1.0	60.0