Hotspot

ndhB-trnL-CAA

IRa · 142925-143466 · 542 bp

Back to Verbena

Pi 0

MarkerSeek score 24.20

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 542
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	TGGCCAGGAAAGAGGGATTA	50.0	50.0	57.9	58.0	1315 bp	1.0	79.9
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	TCTTGAACAGCGAACAACCA	50.0	45.0	57.9	58.0	1131 bp	1.0	79.9
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	602 bp	1.0	79.6
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	600 bp	1.0	79.6
ndhB-trnL-CAA_p5	TTGGTCTACGTACCGGAACT	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.1	58.0	993 bp	1.0	79.3