Hotspot

ycf15-trnL-CAA

IRb · 93459-93822 · 364 bp

Back to Verbena

Pi 0.0192

MarkerSeek score 55.40

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 364
variable_sites: 7
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	TCTTGAACAGCGAACAACCA	AAATGGTAGACACGCGAGAC	45.0	50.0	58.0	58.0	549 bp	1.0	80.7
ycf15-trnL-CAA_p2	TCTTGAACAGCGAACAACCA	TGAAATGGTAGACACGCGAG	45.0	50.0	58.0	58.0	551 bp	1.0	80.7
ycf15-trnL-CAA_p3	TCTTGAACAGCGAACAACCA	AATCTCGTGCTAAACAGCGT	45.0	45.0	58.0	57.9	525 bp	1.0	80.2
ycf15-trnL-CAA_p4	AAAGGAGAAGGAAGGGTGGA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.9	58.0	477 bp	1.0	80.0
ycf15-trnL-CAA_p5	AAGGAGAAGGAAGGGTGGAA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.9	58.0	476 bp	1.0	80.0