Hotspot

ycf4-cemA

LSC · 61573-62408 · 836 bp

Back to Verbena

Pi 0.0145

MarkerSeek score 68.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 12
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTCCGTTGACCCCTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1007-1011 bp	1.0	80.7
ycf4-cemA_p2	TTCCGTTGACCCCTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1006-1010 bp	1.0	80.7
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	933-937 bp	1.0	80.3
ycf4-cemA_p4	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	962-966 bp	1.0	79.4
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.7	58.1	936-940 bp	1.0	78.7