Hotspot

ycf1

SSC · 123512-128896 · 5385 bp

Back to Verbascum

Pi 0.00341

MarkerSeek score 45.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5385
variable_sites: 43
pi: 0.00341
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTTTCTTACAGCCTTGTCA	GCAGAATACCGTCACCCATT	42.9	50.0	57.5	58.0	286-301 bp	1.0	73.4
ycf1_p2	TCGTTTCTTACAGCCTTGTCA	AGGGTGGAAAGTGAGGAAGA	42.9	50.0	57.5	57.9	227-242 bp	1.0	73.1
ycf1_p3	TCGTTTCTTACAGCCTTGTCA	ACGGAAGAAAGGGTGGAAAG	42.9	50.0	57.5	57.7	236-251 bp	1.0	71.8
ycf1_p4	TCGTTTCTTACAGCCTTGTCA	CGGAAGAAAGGGTGGAAAGT	42.9	50.0	57.5	57.7	235-250 bp	1.0	71.8
ycf1_p5	CGTTTCTTACAGCCTTGTCA	GCAGAATACCGTCACCCATT	45.0	50.0	56.0	58.0	285-300 bp	1.0	69.2