Hotspot

rpl16

LSC · 81823-83108 · 1286 bp

Back to Verbascum

Pi 0.00311

MarkerSeek score 60.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1286
variable_sites: 10
pi: 0.00311
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCGTTTTTGTTTCATCCCCA	TTTCTGGCCAATCAAAGCAA	45.0	40.0	57.5	56.4	1406-1417 bp	1.0	64.3
rpl16_p2	GCGTTTTTGTTTCATCCCCA	TTTTCTGGCCAATCAAAGCA	45.0	40.0	57.5	56.4	1407-1418 bp	1.0	64.3
rpl16_p3	TGCGTTTTTGTTTCATCCCC	TTTCTGGCCAATCAAAGCAA	45.0	40.0	57.5	56.4	1407-1418 bp	1.0	64.3
rpl16_p4	TGCGTTTTTGTTTCATCCCC	TTTTCTGGCCAATCAAAGCA	45.0	40.0	57.5	56.4	1408-1419 bp	1.0	64.3
rpl16_p5	GCGTTTTTGTTTCATCCCCA	TCTGGCCAATCAAAGCAATTC	45.0	42.9	57.5	57.4	1404-1415 bp	1.0	64.3